Details

Autor(en) / Beteiligte

• Petrov, V. N.
• Agaeva, E. V.
• Popovkina, O. E.
• Konoplyannikov, A. G.
• Kaplan, M. A.
• Lepekhina, L. A.
• Sayapina, E. V.
• Semenkova, I. V.

Titel

Modifying Effect of Autotransfusion of Mesenchymal Stromal Cells on the Production of Reactive Oxygen Species and Cytokines by Mononuclear Cells in Patients with Chronic Heart Failure

Ist Teil von

• Bulletin of experimental biology and medicine, 2017-12, Vol.164 (2), p.233-240

Ort / Verlag

New York: Springer US

Erscheinungsjahr

2017

Quelle

SpringerLink

Beschreibungen/Notizen

• We studied in vivo modifying effect of autotransfusion of human bone marrow mesenchymal stromal cells on ROS generation and production of cytokines (TNFα,TNFβ, IL-1α, IL-10, IFNγ, and GM-CSF) and PGE 2 by mononuclear cells of patients ( N =21) with chronic heart failure. These parameters were evaluated prior to (control) and after (immediately and on day 14) intravenous administration of stromal cells in doses of 100-200×10 6 . Immediately after autotransfusion, significant increase of in vitro zymosan-induced chemiluminescence of blood mononuclear cells from 10 patients was observed. At later terms after autotransfusion (day 14), inhibition of chemiluminescent activity of blood mononuclear cells was revealed in 50% patients. We discuss possible mechanisms of involvement of transplanted autologous bone marrow mesenchymal stromal cells in reprogramming of blood mononuclear phagocytes from the pro- to anti-inflammatory phenotype under conditions of their in vivo interaction manifesting in transition from activation to inhibition of ROS-producing activity of macrophages and significant suppression of in vitro LPS-induced production of TNFα and GM-CSF by blood mononuclears against the background of significantly elevated TNFβ, IL-10, and IL-1α concentrations.