Details

Autor(en) / Beteiligte

• Salem, Hassan
• Bauer, Eugen
• Kirsch, Roy
• Berasategui, Aileen
• Cripps, Michael
• Weiss, Benjamin
• Koga, Ryuichi
• Fukumori, Kayoko
• Vogel, Heiko
• Fukatsu, Takema
• Kaltenpoth, Martin

Titel

Drastic Genome Reduction in an Herbivore’s Pectinolytic Symbiont

Ist Teil von

• Cell, 2017-12, Vol.171 (7), p.1520-1531.e13

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2017

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Pectin, an integral component of the plant cell wall, is a recalcitrant substrate against enzymatic challenges by most animals. In characterizing the source of a leaf beetle’s (Cassida rubiginosa) pectin-degrading phenotype, we demonstrate its dependency on an extracellular bacterium housed in specialized organs connected to the foregut. Despite possessing the smallest genome (0.27 Mb) of any organism not subsisting within a host cell, the symbiont nonetheless retained a functional pectinolytic metabolism targeting the polysaccharide’s two most abundant classes: homogalacturonan and rhamnogalacturonan I. Comparative transcriptomics revealed pectinase expression to be enriched in the symbiotic organs, consistent with enzymatic buildup in these structures following immunostaining with pectinase-targeting antibodies. Symbiont elimination results in a drastically reduced host survivorship and a diminished capacity to degrade pectin. Collectively, our findings highlight symbiosis as a strategy for an herbivore to metabolize one of nature’s most complex polysaccharides and a universal component of plant tissues. [Display omitted] •Symbiosis is a strategy for an herbivore to gain pectinolytic metabolic enzymes•Stammera has the smallest genome of any known organism not living within a host cell•Specialized structures on the beetle house the symbiont and ensure vertical transfer•Symbiont acquisition likely relaxed selection for the host to endogenously maintain pectinases A proteobacterial symbiont with the smallest known genome of an extracellular bacterium provides its host beetle with key enzymes to break down pectin in plant-based food, giving a striking example of symbiosis and evolutionary adaptation.