Details

Autor(en) / Beteiligte

• Lopes, Marlene
• Gomes, Andreia S.
• Silva, Carla M.
• Belo, Isabel

Titel

Microbial lipids and added value metabolites production by Yarrowia lipolytica from pork lard

Ist Teil von

• Journal of biotechnology, 2018-01, Vol.265, p.76-85

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2018

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• •The oil content (%) was one of the highest reported for a wild Y. lipolytica strain.•Oxygen was an important factor on microbial lipids, lipase and citric acid production.•The unsaturated fatty acids ratio of these lipids is higher than that of pork lard.•Co-production of lipids, citric acid and lipase led to a cost-effective process.•Y. lipolytica acts as a biorefinery to convert pork lard into added value products. Yarrowia lipolytica is a suitable microbial platform to convert low-value hydrophobic substrates into microbial oils and other important metabolites. In this work, this yeast species was used to simultaneously synthetize ex novo lipids and produce citric acid and lipase from animal fat (pork lard) adding higher value to the low-cost fatty substrate. The effect of pH, lard concentration, arabic gum concentration and oxygen mass transfer rate (OTR) on lipids accumulation on Y. lipolytica batch cultures was assessed by an experimental design based on Taguchi method. OTR was by far the most influential parameter in the range of 96mgL−1h−1–480mgL−1h−1. A bio-modification of initial lipidic substrate was observed and, depending on the nutritional and operational conditions, specialty lipids with specific composition and high added-value were obtained. The unsaturated-to-saturated fatty acids ratio of these microbial lipids is higher than in initial substrate, which indicates that they are more suitable than animal fat for food additives. Moreover, the simultaneous induction of lipase and citric acid by Y. lipolytica growing in animal fat demonstrates that a biorefinery approach may be designed based on animal fat raw material.