Details

Autor(en) / Beteiligte

• Quesada‐Gómez, José Manuel
• Santiago‐Mora, Raquel
• Durán‐Prado, Mario
• Dorado, Gabriel
• Pereira‐Caro, Gema
• Moreno‐Rojas, José Manuel
• Casado‐Díaz, Antonio

Titel

β‐Cryptoxanthin Inhibits Angiogenesis in Human Umbilical Vein Endothelial Cells Through Retinoic Acid Receptor

Ist Teil von

• Molecular nutrition & food research, 2018-01, Vol.62 (2), p.n/a

Ort / Verlag

Germany: Wiley Subscription Services, Inc

Erscheinungsjahr

2018

Quelle

MEDLINE

Beschreibungen/Notizen

• Scope β‐Cryptoxanthin is an abundant carotenoid in fruits and vegetables that can be quantified in human blood serum. Yet, contrary to other carotenoids, its effects on endothelial cells and angiogenesis remain unknown. Methods and results Human umbilical vein endothelial cells (HUVEC) are treated with 0.01, 0.1, or 1 μm of β‐cryptoxanthin. Antioxidant activity is determined by its free radical scavenging and oxygen‐radical absorbance capacity. The effect on migration and formation of tubular structures is studied. Additionally, effect on angiogenesis is also analyzed using an in vivo model. β‐Cryptoxanthin exhibits scavenging ability, having an antioxidant effect on HUVEC. Interestingly, β‐cryptoxanthin reduces their migration and angiogenesis, even in the presence of vascular endothelial growth factor (VEGF). Additionally, such carotenoid inhibits in vivo angiogenesis induced by VEGF. In addition, treatment of HUVEC with LE540 (retinoic acid receptor [RAR] panantagonist) inhibits β‐cryptoxanthin antiangiogenic effect on HUVEC. Conclusion β‐Cryptoxanthin inhibits angiogenesis through RAR. Thus, this carotenoid and food containing it may be useful for the prevention and treatment of angiogenic pathologies. That includes tumoral growth and wet macular degeneration associated with aging. To the best of our knowledge, this is the first report of the antioxidant effect and antiangiogenic activity of this carotenoid on HUVEC, both in vitro and in vivo. Currently, little is known about the effect of β‐cryptoxanthin, an abundant carotenoid in fruits and vegetables, on angiogenesis. Interestingly, it is shown in this study that this compound acts as an antioxidant. Thus, it exhibits scavenging ability, decreasing reactive oxygen species levels in human umbilical vein endothelial cells. In these cells, it also reduces migration and angiogenesis, even in the presence of vascular endothelial growth factor (VEGF). Besides, in vivo angiogenesis induced by VEGF was inhibited by β‐cryptoxanthin. In part, this inhibitory effect is mediated by its interaction with retinoic acid receptor. Therefore, it may be useful for prevention and treatment of different pathologies, like the ones related to oxidative stress and angiogenesis, such as cancer.