Details

Autor(en) / Beteiligte

• Movilla, N.
• Borau, C.
• Valero, C.
• García-Aznar, J.M.

Titel

Degradation of extracellular matrix regulates osteoblast migration: A microfluidic-based study

Ist Teil von

• Bone (New York, N.Y.), 2018-02, Vol.107, p.10-17

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2018

Quelle

MEDLINE

Beschreibungen/Notizen

• Bone regeneration is strongly dependent on the capacity of cells to move in a 3D microenvironment, where a large cascade of signals is activated. To improve the understanding of this complex process and to advance in the knowledge of the role of each specific signal, it is fundamental to analyze the impact of each factor independently. Microfluidic-based cell culture is an appropriate technology to achieve this objective, because it allows recreating realistic 3D local microenvironments by taking into account the extracellular matrix, cells and chemical gradients in an independent or combined scenario. The main aim of this work is to analyze the impact of extracellular matrix properties and growth factor gradients on 3D osteoblast movement, as well as the role of cell matrix degradation. For that, we used collagen-based hydrogels, with and without crosslinkers, under different chemical gradients, and eventually inhibiting metalloproteinases to tweak matrix degradation. We found that osteoblast's 3D migratory patterns were affected by the hydrogel properties and the PDGF-BB gradient, although the strongest regulatory factor was determined by the ability of cells to remodel the matrix. •Osteoblast migration in 3D is crucial for bone regeneration•We quantify the migration patterns of human osteoblasts on 3D collagen matrices modified with crosslinkers•Chemical gradients of PDGF present a low effect on osteoblast migration•Crosslinked collagen gels enhance directional HOB migration but do not affect the motility•Osteoblast degradation capacity is essential for migration in 3D