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Identification of volatile sulfur compounds produced by Shigella sonnei using gas chromatography–olfactometry
Ist Teil von
Food control, 2007-02, Vol.18 (2), p.179-182
Ort / Verlag
Elsevier Ltd
Erscheinungsjahr
2007
Link zum Volltext
Quelle
Elsevier ScienceDirect Journals
Beschreibungen/Notizen
This study investigates volatile sulfur compound production by
Shigella sonnei cultured on tryptic soy agar (TSA) and TSA supplemented with 80
μg/ml of the antibiotic rifampicin (TSA-R). Headspace volatiles were trapped and concentrated using solid phase microextraction and identified by gas chromatography–olfactometry (GC–O) and gas chromatography–mass spectroscopy (GC–MS). Results identified two aroma active compounds: methanethiol (MT) and dimethyl sulfide (DMS). Potential pathways of MT and DMS formation were identified as the breakdown of
l-methionine by
l-methionine-α-deamino-γ-mercaptomethane-lyase (METase) and METase-mediated metabolism of methylated
l-methionine, respectively. DMSO, used in TSA-R preparation, may have contributed to an increase in malodor production due to the action of DMSO reductase.