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Molecular detection of Toxoplasma gondii and Neospora caninum in birds from South Africa
Ist Teil von
Acta tropica, 2018-02, Vol.178, p.93-96
Ort / Verlag
Netherlands: Elsevier B.V
Erscheinungsjahr
2018
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
•Tissues of 110 birds from South Africa were tested by PCR.•All samples were negative for N. caninum.•T. gondii was detected in 2.7% of birds (in Red-eyed Dove, Laughing Dove and Southern-Yellow-billed Hornbill).•Sample from Red-eyed Dove was genotyped and characterized as type II.•First detection of T. gondii in tissue of native African birds and first study focusing on N. caninum in birds from SA.
There are not any records on the detection of Toxoplasma gondii and Neospora caninum in tissues of wild birds in the African continent. The aim of the study was to investigate the occurrence of DNA from these protozoan parasites in brain tissue samples collected in years 2014–2015 from 110 wild and domestic birds of 15 orders. Birds came mainly from the province of Limpopo (n=103); the other seven birds came from other five provinces of South Africa. Parasite DNAs were detected by PCR in animal brains. While all samples were negative for N. caninum, T. gondii DNA was detected in three (2.7%) birds: a Red-eyed Dove (Streptopelia semitorquata), a Laughing Dove (S. senegalensis) and a Southern-Yellow-billed Hornbill (Tockus leucomelas), all from Limpopo province. Positive samples were selected for genotyping by a 15 microsatellite markers method in a single multiplex PCR assay. Only the sample from the Red-eyed Dove was successfully genotyped and characterized as type II. This is the first detection of T. gondii in tissue of native African wild birds and the first study focusing on N. caninum in birds from South Africa.