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Recellularization of well‐preserved decellularized kidney scaffold using adipose tissue‐derived stem cells
Journal of biomedical materials research. Part A, 2018-03, Vol.106 (3), p.805-814
Xue, Aibing
Niu, Guangzhu
Chen, Yuan
Li, Kailin
Xiao, Zhiying
Luan, Yun
Sun, Chao
Xie, Xiaoshuai
Zhang, Denglu
Du, Xiaohang
Kong, Feng
Guo, Yanxia
Zhang, Haiyang
Cheng, Guanghui
Xin, Qian
Guan, Yong
Zhao, Shengtian
2018
Details
Autor(en) / Beteiligte
Xue, Aibing
Niu, Guangzhu
Chen, Yuan
Li, Kailin
Xiao, Zhiying
Luan, Yun
Sun, Chao
Xie, Xiaoshuai
Zhang, Denglu
Du, Xiaohang
Kong, Feng
Guo, Yanxia
Zhang, Haiyang
Cheng, Guanghui
Xin, Qian
Guan, Yong
Zhao, Shengtian
Titel
Recellularization of well‐preserved decellularized kidney scaffold using adipose tissue‐derived stem cells
Ist Teil von
Journal of biomedical materials research. Part A, 2018-03, Vol.106 (3), p.805-814
Ort / Verlag
United States: Wiley Subscription Services, Inc
Erscheinungsjahr
2018
Link zum Volltext
Quelle
Wiley Online Library All Journals
Beschreibungen/Notizen
To establish a recellularization kidney model by using adipose tissue‐derived stem cells (ADSCs) as seeding cells and to investigate the growth and differentiation of ADSCs in decellularized kidney scaffolds. ADSCs were isolated using a modified method and then identified using flow cytometry analysis. Osteogenesis and adipogenesis differentiation were performed. Rat kidneys were decellularized using 0.5% sodium dodecyl sulfate. Immunofluorescence, immunohistochemistry, and scanning electron microscope were conducted to examine the scaffold microstructure. The decellularized kidney scaffold was seeded with ADSCs antegrade through the artery or retrograde through the ureter and cultured for 5–10 days. Hematoxylin and eosin staining, immunofluorescence, and immunohistochemistry were applied to assess growth and differentiation of seeding cells within the scaffold. ADSCs populated within the glomerular, vascular, and tubular area of kidney scaffolds. Cells differentiated toward endothelial or tubular cells. Stromal cell‐derived factor 1 promoted cell attachment in the scaffold. These findings suggest that ADSCs can be used as an additional new source of seeding cells within decellularized kidney scaffold. This combination may offer an alternative to donor kidney transplant. In this way, autologous ADSCs can be utilized as seeding cells in cell‐scaffold kidney regeneration for further clinical transplantation. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 805–814, 2018.
Sprache
Englisch
Identifikatoren
ISSN: 1549-3296
eISSN: 1552-4965
DOI: 10.1002/jbm.a.36279
Titel-ID: cdi_proquest_miscellaneous_1955610497
Format
–
Schlagworte
Adipogenesis
,
Adipose tissue
,
adipose tissue‐derived stem cells
,
Autografts
,
Biocompatibility
,
Biomedical materials
,
Cell adhesion
,
Cytometry
,
decellularization
,
decellularized kidney scaffold
,
Differentiation
,
Flow cytometry
,
Immunofluorescence
,
Immunohistochemistry
,
kidney regeneration
,
Kidneys
,
Osteogenesis
,
Regeneration
,
Scaffolds
,
Scanning electron microscopy
,
SDF-1 protein
,
Sodium
,
Sodium dodecyl sulfate
,
Sodium lauryl sulfate
,
Stem cell transplantation
,
Stem cells
,
stromal cell‐derived factor‐1
,
Transplantation
,
Transplants & implants
,
Ureter
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