Details

Autor(en) / Beteiligte

• Jedidi, Iness
• Couturier, Martine
• Thérond, Patrice
• Gardès-Albert, Monique
• Legrand, Alain
• Barouki, Robert
• Bonnefont-Rousselot, Dominique
• Aggerbeck, Martine

Titel

Cholesteryl ester hydroperoxides increase macrophage CD36 gene expression via PPARα

Ist Teil von

• Biochemical and biophysical research communications, 2006-12, Vol.351 (3), p.733-738

Ort / Verlag

Elsevier Inc

Erscheinungsjahr

2006

Quelle

Elsevier ScienceDirect Journals

Beschreibungen/Notizen

• The uptake of oxidized LDL by macrophages is a key event in the development of atherosclerosis. The scavenger receptor CD36 is one major receptor that internalizes oxidized LDL. In differentiated human macrophages, we compared the regulation of CD36 expression by copper-oxidized LDL or their products. Only oxidized derivatives of cholesteryl ester (CEOOH) increased the amount of CD36 mRNA (2.5-fold). Both oxidized LDL and CEOOH treatment increased two to fourfold the transcription of promoters containing peroxisome-proliferator-activated-receptor responsive elements (PPRE) in the presence of PPARα or γ. Electrophoretic-mobility-shift-assays with nuclear extracts prepared from macrophages treated by either oxidized LDL or CEOOH showed increased binding of PPARα to the CD36 gene promoter PPRE. In conclusion, CEOOH present in oxidized LDL increase CD36 gene expression in a pathway involving PPARα.