Details

Autor(en) / Beteiligte

• Xi, Lijun
• Qiao, Nenghu
• Zhang, Jingjing
• Li, Jing
• Liu, Dejian
• You, Jing
• Liu, Jianguo

Titel

Deinococcus petrolearius sp. nov. isolated from crude oil recovery water in China

Ist Teil von

• Antonie van Leeuwenhoek, 2018-03, Vol.111 (3), p.353-360

Ort / Verlag

Cham: Springer International Publishing

Erscheinungsjahr

2018

Quelle

SpringerLink

Beschreibungen/Notizen

• A Gram-stain positive, non-motile, spherical, red-pigmented and facultatively anaerobic bacterium, designated strain 6.1 T , was isolated from a crude oil recovery water sample from the Huabei oil field in China. The novel strain exhibited tolerance of UV irradiation (> 1000 J m −2 ). Based on 16S rRNA gene sequence comparisons, strain 6.1 T shows high similarity to Deinococcus citri DSM 24791 T (98.1%) and Deinococcus gobiensis I-0 T (97.8%), with less than 93.5% similarity to other closely related taxa. The major cellular fatty acids were identified as summed feature 3 (C 16:1 ω 7 c and/or iso-C 15:0 2-OH), followed by iso-C 17:1 ω 9 c and C 16:0 . The polar lipid profile was found to contain phospholipids, glycolipids, phosphoglycolipids and aminophospholipids. The predominant respiratory quinone was identified as MK-8. The DNA G + C content was determined to be 68.3 mol %. DNA–DNA hybridization between strain 6.1 T and D. citri DSM 24791 T was 45.6 ± 7.1% and with D. gobiensis I-O T was 36.6 ± 4.7%. On the basis of phylogenetic, chemotaxonomic and phenotypic data, we conclude strain 6.1 T represents a novel species of the genus Deinococcus , for which we propose the name Deinococcus petrolearius sp. nov. The type strain is 6.1 T (= CGMCC 1.15053 T  = KCTC 33744 T ).