Biochemistry (Moscow), 2017-08, Vol.82 (8), p.933-941
2017
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- Autor(en) / Beteiligte
- Titel
- Nigericin inhibits epithelial ovarian cancer metastasis by suppressing the cell cycle and epithelial−mesenchymal transition
- Ist Teil von
- Biochemistry (Moscow), 2017-08, Vol.82 (8), p.933-941
- Ort / Verlag
- Moscow: Pleiades Publishing
- Erscheinungsjahr
- 2017
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Epithelial ovarian cancer (EOC) has the highest mortality among various types of gynecological malignancies. Most patients die of metastasis and recurrence due to cisplatin resistance. Thus, it is urgent to develop novel therapies to cure this disease. CCK-8 assay showed that nigericin exhibited strong cytotoxicity on A2780 and SKOV3 cell lines. Flow cytometry indicated that nigericin could induce cell cycle arrest at G0/G1 phase and promote cell apoptosis. Boyden chamber assay revealed that nigericin could inhibit migration and invasion in a dose-dependent manner by suppressing epithelial–mesenchymal transition (EMT) in EOC cells. These effects were mediated, at least partly, by the Wnt/β-catenin signaling pathway. Our results demonstrated that nigericin could inhibit EMT during cell invasion and metastasis through the canonical Wnt/β-catenin signaling pathway. Nigericin may prove to be a novel therapeutic strategy that is effective in patients with metastatic EOC.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0006-2979eISSN: 1608-3040DOI: 10.1134/S0006297917080089Titel-ID: cdi_proquest_miscellaneous_1942717264
- Format
- –
- Schlagworte
- Antibiotics, Antineoplastic Agents - pharmacology, Apoptosis, Apoptosis - drug effects, Biochemistry, Biomedical and Life Sciences, Biomedicine, Bioorganic Chemistry, Biotechnology, Boyden chamber, Cancer, Cancer metastasis, Carcinoma, Ovarian Epithelial, Cell cycle, Cell Line, Tumor, Cell Movement - drug effects, Cell Proliferation - drug effects, Cholecystokinin, Cisplatin, Cytometry, Cytotoxicity, Dosage and administration, Epithelial-Mesenchymal Transition - drug effects, Female, Flow cytometry, G1 phase, G1 Phase Cell Cycle Checkpoints - drug effects, Humans, Life Sciences, Mesenchyme, Metastases, Metastasis, Microbiology, Microscopy, Fluorescence, Neoplasms, Glandular and Epithelial - metabolism, Neoplasms, Glandular and Epithelial - pathology, Nigericin, Nigericin - pharmacology, Ovarian cancer, Ovarian Neoplasms - metabolism, Ovarian Neoplasms - pathology, Patients, Prevention, Retarding, Signal transduction, Toxicity, Wnt protein, Wnt Signaling Pathway - drug effects, β-Catenin