Digestive diseases and sciences, 2013-07, Vol.58 (7), p.1909-1915
2013
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- Autor(en) / Beteiligte
- Titel
- γ-Aminobutyric Acid B Receptor Improves Carbon Tetrachloride-Induced Liver Fibrosis in Rats
- Ist Teil von
- Digestive diseases and sciences, 2013-07, Vol.58 (7), p.1909-1915
- Ort / Verlag
- Boston: Springer US
- Erscheinungsjahr
- 2013
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Background It was well known that angiotension II can inhibit hepatic stellate cell activation. The GABA B receptor was upregulated when the hepatic stellate cell line was stimulated by angiotension II in our previous study. But the role of the GABA B receptor in liver fibrosis has never been reported. Aim In the present study, we investigated the effects of this receptor on carbon tetrachloride-induced liver fibrosis in rats. Methods The rats were divided into four groups including GABA B receptor agonist, antangonist, model and control group. α-smooth muscle actin (α-SMA) and GABA B receptor expression levels were detected by immunohistochemistry and real-time polymerase chain reaction. Liver function tests were performed once blood samples was taken; Western blot analysis was used to detect protein expression level of α-SMA and TGF-β1. Results We found baclofen ameliorated the CCl 4 -induced rats’s liver fibrosis. The highest liver enzymes and α-SMA protein levels were found in the CGP35348 group. Conclusion The GABA B receptor may have a protective role in the liver.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0163-2116eISSN: 1573-2568DOI: 10.1007/s10620-013-2623-zTitel-ID: cdi_proquest_miscellaneous_1942222038
- Format
- –
- Schlagworte
- Actins - metabolism, Animals, Baclofen - pharmacology, Baclofen - therapeutic use, Biochemistry, Biomarkers - metabolism, Blotting, Western, Carbon Tetrachloride, Drug Administration Schedule, GABA-B Receptor Agonists - pharmacology, GABA-B Receptor Agonists - therapeutic use, GABA-B Receptor Antagonists - pharmacology, GABA-B Receptor Antagonists - therapeutic use, Gastroenterology, Hepatology, Immunohistochemistry, Liver - drug effects, Liver - metabolism, Liver - pathology, Liver - physiopathology, Liver Cirrhosis - chemically induced, Liver Cirrhosis - drug therapy, Liver Cirrhosis - metabolism, Liver Function Tests, Male, Medicine, Medicine & Public Health, Oncology, Original Article, Rats, Rats, Sprague-Dawley, Real-Time Polymerase Chain Reaction, Receptors, GABA-B - metabolism, Reverse Transcriptase Polymerase Chain Reaction, Transforming Growth Factor beta1 - metabolism, Transplant Surgery