Details

Autor(en) / Beteiligte

• Raeven, R Hm
• Brummelman, J
• Pennings, J L A
• van der Maas, L
• Helm, K
• Tilstra, W
• van der Ark, A
• Sloots, A
• van der Ley, P
• van Eden, W
• Jiskoot, W
• van Riet, E
• van Els, C Acm
• Kersten, G Fa
• Han, W Gh
• Metz, B

Titel

Molecular and cellular signatures underlying superior immunity against Bordetella pertussis upon pulmonary vaccination

Ist Teil von

• Mucosal immunology, 2018-05, Vol.11 (3), p.979-993

Ort / Verlag

United States: Nature Publishing Group

Erscheinungsjahr

2018

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Mucosal immunity is often required for protection against respiratory pathogens but the underlying cellular and molecular mechanisms of induction remain poorly understood. Here, systems vaccinology was used to identify immune signatures after pulmonary or subcutaneous immunization of mice with pertussis outer membrane vesicles. Pulmonary immunization led to improved protection, exclusively induced mucosal immunoglobulin A (IgA) and T helper type 17 (Th17) responses, and in addition evoked elevated systemic immunoglobulin G (IgG) antibody levels, IgG-producing plasma cells, memory B cells, and Th17 cells. These adaptive responses were preceded by unique local expression of genes of the innate immune response related to Th17 (e.g., Rorc) and IgA responses (e.g., Pigr) in addition to local and systemic secretion of Th1/Th17-promoting cytokines. This comprehensive systems approach identifies the effect of the administration route on the development of mucosal immunity, its importance in protection against Bordetella pertussis, and reveals potential molecular correlates of vaccine immunity to this reemerging pathogen.