Details

Autor(en) / Beteiligte

• Agrawal, Veena
• Sardar, Pratima Rani
• Subramanian, J.

Titel

In vitro regeneration through somatic embryogenesis and organogenesis using cotyledons of Cassia angustifolia Vahl

Ist Teil von

• In vitro cellular & developmental biology. Plant, 2007-12, Vol.43 (6), p.585-592

Ort / Verlag

Heidelberg: The Society for In Vitro Biology

Erscheinungsjahr

2007

Quelle

SpringerLink

Beschreibungen/Notizen

• In vitro regeneration through somatic embryogenesis as well as organogenesis using cotyledon of a woody medicinal legume, Cassia angustifolia is reported. The cotyledons dissected from semi-mature seeds, if inoculated on Murashige and Skoog's medium (MS) supplemented with auxin alone or in combination with cytokinin, produced direct and indirect somatic embryos. A maximum of 14.36±2.26 somatic embryos per 20 mg of explants including callus were produced in 70% cultures on MS medium with 2.5 μM benzyladenine (BA) + 10 μM 2,4-dichlorophenoxyacetic acid (2,4-D). Although the percentage of embryogenic cultures was higher (83.33%) at 10 μM 2,4-D + 1 μM BA, the average number of somatic embryos was much less (7.6±0.85) at this level, whereas at 2.5 μM BA and 5 μM 2,4-D, there was a simultaneous formation of both somatic embryos and shoots. The somatic embryos, although started germinating on the same medium, developed into full plantlets only if transferred to MS basal with 2% sucrose. Cytokinins alone did not induce somatic embryogenesis, but formed multiple shoots. Five micromolar BA proved optimum for recurrently inducing shoots in the competent callus with a maximum average of 12.04±2.10 shoots and shoot length of 2.26±0.03 cm. Nearly 91.6% shoots (2–2.5 cm in size) organized an average of 5.12±0.58 roots on half strength MS + 10 μM indole-3-butyric acid. All the plantlets have been transferred successfully to soil. Types of auxin and its interaction with cytokinin significantly influenced somatic embryogenesis.