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Mycocinotyping of cryptococci from the Prioksko-terrasny reserve (Russia) has revealed three strains that have a unique mycocin-sensitivity profile. Sequencing results of the D1/D2 domain of the 26S rDNA and of the complete ITS region placed them in the
Floriforme clade of the Filobasidiales lineage (Hymenomycetes). The three strains had identical sequences, which differed from those of known
Filobasidium and
Cryptococcus species. A novel species named
Cryptococcus stepposus (type strain VKM Y-2918) is proposed to accommodate these isolates. Physiological characteristics distinguishing the novel species from other
Cryptococcus species in the
Floriforme clade are presented.