Details

Autor(en) / Beteiligte

• Ohhata, T.
• Hoki, Y.
• Sasaki, H.
• Sado, T.

Titel

Tsix-deficient X chromosome does not undergo inactivation in the embryonic lineage in males: implications for Tsix-independent silencing of Xist

Ist Teil von

• Cytogenetic and genome research, 2006-03, Vol.113 (1-4), p.345-349

Ort / Verlag

Basel, Switzerland: S. Karger AG

Erscheinungsjahr

2006

Quelle

MEDLINE

Beschreibungen/Notizen

• Differential induction of the X-linked non-coding Xist gene is a key event in the process of X inactivation occurring in female mammalian embryos. Xist is negatively regulated in cis by its antisense gene Tsix through modification of the chromatin structure. The maternal Xist allele, which is normally silent in the extraembryonic lineages, is ectopically activated when Tsix is disrupted on the same chromosome, and subsequently the maternal X chromosome undergoes inactivation in the extraembryonic lineages even in males. However, it is still unknown whether the single Tsix-deficient X chromosome (X Δ Tsix ) in males is also inactivated in the embryonic lineage. Here, we show that both male and female embryos carrying a maternally derived X Δ Tsix could survive if the extraembryonic tissues were complemented by wild-type tetraploid cells. In addition, Xist on the X Δ Tsix was properly silenced and methylated at CpG sites in adult male somatic cells. These results indicate that the embryonic lethality caused by the maternal X Δ Tsix is solely attributable to the defects in the extraembryonic lineages. X Δ Tsix does not seem to undergo inactivation in the embryonic lineage in males, suggesting the presence of a Tsix-independent silencing mechanism for Xist in the embryonic lineage.