Details

Autor(en) / Beteiligte

• Choi, In-Young
• Moon, Phil-Dong
• Koo, Hyun-Na
• Myung, Noh-Yil
• Kim, Su-Jin
• Lee, Ji-Hyun
• Han, Se-Hee
• Moon, Goo
• Seo, Sung-Yum
• Sung, Hyun-Jea
• Park, Rae-Kil
• Jeong, Hyun-Ja
• Um, Jae-Young
• Kim, Hyung-Min
• Hong, Seung-Heon
• Sato, J. Denry

Titel

Observations of Forsythia koreana methanol extract on mast cell-mediated allergic reactions in experimental models

Ist Teil von

• In vitro cellular & developmental biology. Animal, 2007-07, Vol.43 (7), p.215-221

Ort / Verlag

Germany: The Society for In Vitro Biology 2007

Erscheinungsjahr

2007

Quelle

MEDLINE

Beschreibungen/Notizen

• To explore effects of Forsythia koreana methanol extract (FKME) on mast cell-mediated allergic and inflammatory properties, the effect of FKME was evaluated on compound 48/80-induced systemic anaphylaxis, ear swelling, and anti-dinitrophenyl (DNP) immunoglobulin E (IgE)-induced passive cutaneous anaphylaxis (PCA). In addition, the effect of FKME was investigated on the histamine release from rat peritoneal mast cells (RPMCs) stimulated by compound 48/80, which promotes histamine release. The human mast cell line HMC-1 was stimulated by phorbol 12-myristate 13-acetate plus calcium ionophore A23187. Activated HMC-1 can produce several proinflammatory and chemotactic cytokines such as tumor necrosis factor-alpha (TNF-α), interleukin (IL)-6, and IL-8. Cytokine levels in the culture supernatant were measured by an enzyme-linked immunosorbent assay. Cytotoxicity by FKME was determined by a 3-(4,5-dimethylthiazol-2-yl)-diphenyl-tetrazolium bromide (MTT) assay. FKME inhibited compound 48/80-induced systemic anaphylactic shock and ear swelling in mice. When 1 g/kg FKME was pretreated or posttreated with mice, compound 48/80-induced mice morality was 50 and 66.7%, respectively. One gram per kilogram of FKME pretreatment inhibited ear-swelling responses derived from compound 48/80 by 29.75%. A PCA reaction was inhibited by 17.9%. In an in vitro model, FKME (1 mg/ml) inhibited histamine release from the RPMCs by 13.8% and TNF-α, IL-6, and IL-8 production from HMC-1 cells by 71.16% (P<0.001), 86.72% (P<0.001), and 44.6%, respectively. However, FKME had no cytotoxic effects on cell viability. In conclusion, FKME inhibited not only systemic anaphylaxis and ear swelling induced by compound 48/80 but also inhibited a PCA reaction induced by anti-DNP IgE in vivo. Treatment with FKME showed significant inhibitory effects on histamine, TNF-α, IL-6, and IL-8 release from mast cells.