Details

Autor(en) / Beteiligte

• Utsumi, Takako
• Lusida, Maria Inge
• Dinana, Zayyin
• Wahyuni, Rury Mega
• Yamani, Laura Navika
• Juniastuti
• Soetjipto
• Matsui, Chieko
• Deng, Lin
• Abe, Takayuki
• Doan, Yen Hai
• Fujii, Yoshiki
• Kimura, Hirokazu
• Katayama, Kazuhiko
• Shoji, Ikuo

Titel

Occurrence of norovirus infection in an asymptomatic population in Indonesia

Ist Teil von

• Infection, genetics and evolution, 2017-11, Vol.55, p.1-7

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• Norovirus (NoV) is a major cause of nonbacterial acute gastroenteritis worldwide in all age groups, and asymptomatic individuals may contribute to NoV transmission as a reservoir. Nonetheless, little information is available regarding asymptomatic NoV infection in Indonesia. We performed an epidemiological analysis of NoV infection among asymptomatic healthy volunteers in the city of Surabaya, Indonesia (population ~2.75 million). A total of 512 stool samples from 18 individuals (age range 20–42years) collected from July 2015 to June 2016 were examined. The detection of NoV and the genotype classification were carried out by a reverse transcription-polymerase chain reaction (RT-PCR) direct sequencing method. NoV was detected in 14 of the 512 stool samples (2.7%), with 7 individuals (38.9%) having at least 1 positive stool sample. All 14 of the NoV strains detected belonged to genogroup GII. The phylogenetic analysis indicated that 10 strains (71.4%) were grouped with GII.2, 2 (14.3%) were GII.17, 1 was GII.4 Sydney 2012, and 1 was GII.1. The circulation of GII.Pg/GII.1 and GII.Pe/GII.4 Sydney 2012 recombinant variants was detected among an asymptomatic population in Surabaya, Indonesia. Of the 7 positive individuals, 2 were repeatedly infected with the same strain and heterogenous strains. Taken together, our results suggest that the excretion of NoV from healthy individuals is one of the sources of NoV outbreak. •An epidemiological study of norovirus infection was performed in healthy volunteers.•Stool samples were collected from 18 Indonesian individuals during 2015 to 2016.•Norovirus was detected in 14 of 512 stool samples (7 individuals).•The predominant genotype among healthy individuals was GII.2 in the capsid region.•GII.Pg/GII.1 and GII.Pe/GII.4 Sydney 2012 recombinant variants were detected.