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Multicolor flow cytometric analysis of TLR2 and TLR9 expression and function in NK cells from patients with ANCA‐associated vasculitis
Cytometry. Part B, Clinical cytometry, 2018-05, Vol.94 (3), p.412-422
Scrivo, Rossana
Peruzzi, Giovanna
Gattamelata, Angelica
Gross, Catharina C.
Carletti, Raffaella
Di Gioia, Cira
Brandt, Jessica
Priori, Roberta
Morrone, Stefania
Santoni, Angela
Valesini, Guido
2018
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Scrivo, Rossana
Peruzzi, Giovanna
Gattamelata, Angelica
Gross, Catharina C.
Carletti, Raffaella
Di Gioia, Cira
Brandt, Jessica
Priori, Roberta
Morrone, Stefania
Santoni, Angela
Valesini, Guido
Titel
Multicolor flow cytometric analysis of TLR2 and TLR9 expression and function in NK cells from patients with ANCA‐associated vasculitis
Ist Teil von
Cytometry. Part B, Clinical cytometry, 2018-05, Vol.94 (3), p.412-422
Ort / Verlag
United States: Wiley Subscription Services, Inc
Erscheinungsjahr
2018
Quelle
MEDLINE
Beschreibungen/Notizen
Background The primary objective of this study was to provide an assessment of NK cells in patients with ANCA‐associated vasculitis (AAV). Methods Patients were classified based on the presence or absence of ANCAs and compared with healthy controls (HCs). By multiparameter flow cytometry, we evaluated the number and proportion of NK cells (CD3‐CD56+) and the CD56dim, CD56bright, CD56dimCD57bright subsets; TLR2 and TLR9 expression; intracellular IFN‐γ production upon stimulation with TLR2 and TLR9 ligands; degranulation activity; serum cytokines; immunohistochemical staining of available biopsies. Results No differences in the number and proportion of NK cells between patients and HC were observed, except for a lower proportion of CD56dim subset in ANCA‐negative patients than in HC (P = 0.02). With respect to HC, TLR2 expression levels were reduced in NK cells from ANCA‐negative patients (P = 0.03), in the CD56dim subset of ANCA‐positive (P = 0.02) and ANCA‐negative patients (P = 0.01), in the CD56bright subset of ANCA‐positive patients (P = 0.007), and in the CD56dimCD57bright subset of ANCA‐positive (P = 0.04) and ANCA‐negative patients (P = 0.03). No differences between patients and HC were found concerning IFN‐γ production and degranulation activity. IL‐22 levels were lower in ANCA‐positive patients than in HC (P = 0.01). The immunohistochemical analysis showed sporadic CD56+ cells in one renal biopsy, and a diffuse and moderate infiltrate of IL‐22+ cells in all renal biopsies and in skin tissue. Conclusions Our data suggest a role of infectious stimuli triggering NK cells in AAV pathogenesis. Poor detection of NK cells in affected tissues suggests a marginal involvement in local inflammatory responses. © 2017 International Clinical Cytometry Society
Sprache
Englisch
Identifikatoren
ISSN: 1552-4949
eISSN: 1552-4957
DOI: 10.1002/cyto.b.21586
Titel-ID: cdi_proquest_miscellaneous_1932165954
Format
–
Schlagworte
Aged
,
ANCA
,
vasculitis
,
ANCA‐associated vasculitis
,
NK cells
,
TLRs
,
Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis - metabolism
,
Antineutrophil cytoplasmic antibodies
,
Biopsy
,
Case-Control Studies
,
CD3 antigen
,
CD56 antigen
,
CD56 Antigen - metabolism
,
Cytokines
,
Degranulation
,
Female
,
Flow cytometry
,
Flow Cytometry - methods
,
Humans
,
Inflammation
,
Interferon
,
Interferon-gamma - metabolism
,
Killer Cells, Natural - metabolism
,
Leukocytes, Mononuclear - metabolism
,
Male
,
Middle Aged
,
Pathogenesis
,
Patients
,
Skin
,
TLR2 protein
,
TLR9 protein
,
Toll-Like Receptor 2 - metabolism
,
Toll-Like Receptor 9 - metabolism
,
Toll-like receptors
,
Vasculitis
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