Details

Autor(en) / Beteiligte

• Li, Duan-Duan
• Shi, Shan-Hui
• Zhou, Mi
• Ma, Xiu-Xia
• Gao, Jian-Zhong
• Zhang, Ya-Qiong
• Yang, Li-Wen
• Zuo, Lin

Titel

Optimization of isolation and culture of Lin - Sca-1 + cardiac stem cells from newborn mice

Ist Teil von

• Sheng li hsüeh pao, 2017-08, Vol.69 (4), p.477-484

Ort / Verlag

China

Erscheinungsjahr

2017

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Cardiac stem cells (CSCs) transplantation has been recognized to be effective on the treatment of myocardial infarction (MI), but some techniques still need to be developed in the isolation and culture of CSCs, which is the key problem restricting the clinical application of CSCs. This study was focused on the isolation of Lin (lineage-negative) Sca-1 (stem cell antigen-1-positive) CSCs from newborn C57BL/6J mice (0-3 d) by mixed enzymatic-explant isolation in combination with immunomagnetic separation. The digesting time, digesting frequency, incubation temperature, stirring speed, centrifugation time and rotational speed were strictly controlled in the experiment. In order to increase the survival rate of CSCs, the medium changing time and manner were optimized in primary CSCs culture. The percentages of Sca-1 cells in primary and passage cells were detected by flow cytometry and immunofluorescence staining. The results showed that: (1) the proportion of Lin Sca-1 cells within the collected cells could be a