Details

Autor(en) / Beteiligte

• Kuroiwa, H
• Yoshida, Y
• Nishida, K
• Misumi, O
• Yagisawa, F
• Fujiwara, T
• Kuroiwa, F

Titel

Analysis on the structure and function of the chloroplast division apparatus in primitive red alga Cyanidioschyzon merolae

Ist Teil von

• Journal of plant research, 2006-12, Vol.119, p.124-124

Erscheinungsjahr

2006

Quelle

Springer Nature - Complete Springer Journals

Beschreibungen/Notizen

• About the chloroplast division mechanism, it became obvious that the chloroplast fission was driven by chloroplast division rings such as the Dynamic trio: FtsZ ring, Dynamin ring and the plastid-dividing (PD) rings. Past several years, the structure and the behavior of these rings were investigated. FtsZ, which was a plant homolog of the key bacterial division protein, appears at an earlier stage of the constriction at the inside of the chloroplast inner membrane, forming the ring apparatus and disappears at the last stage of the constriction. While, eukaryote specific protein, Dynamin, assembles and forms the ring structure at the outside of the outer membrane at the middle to later stage, and finally enters to the inner site of the PD ring in the last stage of the constriction. Thus, these 3 rings perform the chloroplast division, carrying out the different functions each other (Kuroiwa et al. 1998, Miyagishima et al. 2001 similar to 2003). In this study, we tried to isolate the intact chloroplast division apparatus from a red alga, Cyanidioschyzon merolae. Immuno- electron microscopy showed that the isolated apparatus contained both FtsZ protein and dynamin protein along PD rings, indicating the formation of the compound apparatus. We analyze the existence pattern of these rings and presume the relation between PD ring and these rings.