Details

Autor(en) / Beteiligte

• Sönmez, Tolga Taha
• Bayer, Andreas
• Cremer, Tillman
• Hock, Jennifer Vanessa Phi
• Lethaus, Bernd
• Kweider, Nisreen
• Wruck, Christoph Jan
• Drescher, Wolf
• Jahr, Holger
• Lippross, Sebastian
• Pufe, Thomas
• Tohidnezhad, Mersedeh

Titel

The protective effect of platelet released growth factors and bone augmentation (Bio-Oss®) on ethanol impaired osteoblasts

Ist Teil von

• Annals of anatomy, 2017-11, Vol.214, p.36-42

Ort / Verlag

Germany: Elsevier GmbH

Erscheinungsjahr

2017

Quelle

ScienceDirect Pay Per View(PPV) Titles

Beschreibungen/Notizen

• Chronic alcohol consumption is a known limiting factor for bone healing. One promising strategy to improve bone augmentation techniques with Bio-Oss® in oral and maxillofacial surgery might be the supportive application of platelet-concentrated biomaterials as platelet-released growth factor (PRGF). To address this matter, we performed an in vitro study investigating the protective effects of PRGF and Bio-Oss® in ethanol (EtOH) treated osteoblasts. The SAOS-2 osteosarcoma cell line, with and without EtOH pretreatment was used. The cell viability, proliferation and alkali phosphatase activity (ALP) after application of 0%, 5% and 10% PRGF and Bio-Oss® were assessed. The application of PRGF and Bio-Oss® in EtOH impaired osteoblasts showed a significant beneficial influence increasing the viability of the osteoblasts in cell culture. The synergistic effect of Bio-Oss® and 5% PRGF on the proliferation of osteoblasts was also demonstrated. Bio-Oss® only in combination with PRGF increases the alkaline phosphatase (ALP) activity in EtOH pretreated cells. These results indicate that the simultaneous application of PRGF and Bio-Oss® inhibits EtOH induced bone healing impairment. Furthermore, in the cells, PRGF induced a protective mechanism which might promote bone regeneration.