Details

Autor(en) / Beteiligte

• Davids, Madars
• Gudra, Dita
• Radovica-Spalvina, Ilze
• Fridmanis, Davids
• Bartkevics, Vadims
• Muter, Olga

Titel

The effects of ibuprofen on activated sludge: Shift in bacterial community structure and resistance to ciprofloxacin

Ist Teil von

• Journal of hazardous materials, 2017-10, Vol.340, p.291-299

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2017

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• [Display omitted] •Ibuprofen (IBP) above 50mg/L inhibits an oxygen demand in the activated sludge (AS).•Selective pressure of IBP led to dominance of α-Proteobacteria in AS.•Eosin Methylene Blue was more sensitive for susceptibility test than Nutrient Agar.•High concentrations of IBP could increase the ciprofloxacin-resistance in AS. Ibuprofen (IBP) is ranked at the 4th place among 57 pharmaceutical compounds according to the number of citations in prioritization documents. The response of microbial community of activated sludge to IBP was studied at the concentrations of 50–5000mg/L. Batch incubation was performed in an OxiTop® device for 21days. The reduction of biological oxygen demand depended on the IBP concentration and varied in the range from 321 to 107mg O2/L. Massive DNA sequencing analysis of the activated sludge revealed that Proteobacteria became more dominant when grown in the presence of IBP. Microbial diversity was reduced in the presence of 500–1000mg/L IBP, but increased again in the presence of 5000mg/L IBP, despite the domination of Enterobacteriales (48.1%) in this sample. Incubation of activated sludge in the presence of 1000mg/L IBP led to an increased occurrence of ciprofloxacin-resistant bacteria. The use of Eosin Methylene Blue Agar for disc diffusion assay was shown to be more appropriate in order to reveal the changes in antibiotic resistance. The predominance of Enterobacteriales in the activated sludge is suggested as one of the possible explanations of the enhanced resistance to ciprofloxacin.