Details

Autor(en) / Beteiligte

• Liu, Xiaoyi
• Liu, Yaoping
• Zhao, Feng
• Hun, Tingting
• Li, Shan
• Wang, Yuguang
• Sun, Weijie
• Wang, Wei
• Sun, Yan
• Fan, Yubo

Titel

Regulation of cell arrangement using a novel composite micropattern

Ist Teil von

• Journal of biomedical materials research. Part A, 2017-11, Vol.105 (11), p.3093-3101

Ort / Verlag

United States: Wiley Subscription Services, Inc

Erscheinungsjahr

2017

Link zum Volltext

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• Micropatterning technique has been used to control single cell geometry in many researches, however, this is no report that it is used to control multicelluar geometry, which not only control single cell geometry but also organize those cells by a certain pattern. In this work, a composite protein micropattern is developed to control both cell shape and cell location simultaneously. The composite micropattern consists of a central circle 15 μm in diameter for single‐cell capture, surrounded by small, square arrays (3 μm × 3 μm) for cell spreading. This is surrounded by a border 2 μm wide for restricting cell edges. The composite pattern results in two‐cell and three‐cell capture efficiencies of 32.1% ± 1.94% and 24.2% ± 2.89%, respectively, representing an 8.52% and 9.58% increase, respectively, over rates of original patterns. Fluorescent imaging of cytoskeleton alignment demonstrates that actin is gradually aligned parallel to the direction of the entire pattern arrangement, rather than to that of a single pattern. This indicates that cell arrangement is also an important factor in determining cell physiology. This composite micropattern could be a potential method to precisely control multi‐cells for cell junctions, cell interactions, cell signal transduction, and eventually for tissue rebuilding study. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3093–3101, 2017.