Details

Autor(en) / Beteiligte

• Li, Shao-Jun
• Yong Li
• Jing-Wen Chen
• Zong-Xiang Yuan
• Yu-Huan Mo
• Guo-Dong Lu
• Yue-Ming Jiang
• Chao-Yan Ou
• Fang Wang
• Xiao-Wei Huang
• Yi-Ni Luo
• Shi-Yan Ou
• Yan-Ni Huang

Titel

Sodium Para-aminosalicylic Acid Protected Primary Cultured Basal Ganglia Neurons of Rat from Manganese-Induced Oxidative Impairment and Changes of Amino Acid Neurotransmitters

Ist Teil von

• Biological trace element research, 2016-04, Vol.170 (2), p.357-365

Ort / Verlag

New York: Springer US

Erscheinungsjahr

2016

Quelle

MEDLINE

Beschreibungen/Notizen

• Manganese (Mn), an essential trace metal for protein synthesis and particularly neurotransmitter metabolism, preferentially accumulates in basal ganglia. However, excessive Mn accumulation may cause neurotoxicity referred to as manganism. Sodium para-aminosalicylic acid (PAS-Na) has been used to treat manganism with unclear molecular mechanisms. Thus, we aim to explore whether PAS-Na can inhibit Mn-induced neuronal injury in basal ganglia in vitro. We exposed basal ganglia neurons with 50 μM manganese chloride (MnCl₂) for 24 h and then replaced with 50, 150, and 450 μM PAS-Na treatment for another 24 h. MnCl₂ significantly decreased cell viability but increased leakage rate of lactate dehydrogenase and DNA damage (as shown by increasing percentage of DNA tail and Olive tail moment). Mechanically, Mn reduced glutathione peroxidase and catalase activity and interrupted amino acid neurotransmitter balance. However, PAS-Na treatment reversed the aforementioned Mn-induced toxic effects. Taken together, these results showed that PAS-Na could protect basal ganglia neurons from Mn-induced neurotoxicity.