Details

Autor(en) / Beteiligte

• Ho, C.‐C.
• Fang, H.‐Y.
• Wang, B.
• Huang, T.‐H.
• Shie, M.‐Y.

Titel

The effects of Biodentine/polycaprolactone three‐dimensional‐scaffold with odontogenesis properties on human dental pulp cells

Ist Teil von

• International endodontic journal, 2018-05, Vol.51 (S4), p.e291-e300

Ort / Verlag

England: Wiley Subscription Services, Inc

Erscheinungsjahr

2018

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Aim To determine the feasibility of using three‐dimensional printed Biodentine/polycaprolactone composite scaffolds for orthopaedic and dental applications. The physicochemical properties and the odontogenic differentiation of human dental pulp cells (hDPCs) were investigated. Methodology Biodentine was well‐suspended in ethanol and dropped slowly into molten polycaprolactone with vigorous stirring. The Biodentine/polycaprolactone composite scaffolds were then fabricated into controlled macropore sizes and structures using an extrusion‐based three‐dimensional (3D) printer. The mechanical properties, bioactivity, and the proliferation and odontogenic differentiation of human dental pulp cells (hDPCs) cultured on the scaffolds were evaluated. Results Biodentine/polycaprolactone scaffolds had uniform macropores 550 μm in size with established interconnections and a compressive strength of 6.5 MPa. In addition, the composite scaffolds exhibited a good apatite‐forming ability and were capable of supporting the proliferation and differentiation of hDPCs. Conclusion The composite scaffolds fabricated by an extrusion‐based 3D printing technique had similar characteristics to Biodentine cement, including bioactivity and the ability to promote the differentiation of hDPCs. These results indicate that the composite scaffold would be a candidate for dental and bone regeneration.