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Labeling and Magnetic Resonance Imaging of Exosomes Isolated from Adipose Stem Cells
Current protocols in cell biology, 2017-06, Vol.75 (1), p.3.44.1-3.44.15
Busato, Alice
Bonafede, Roberta
Bontempi, Pietro
Scambi, Ilaria
Schiaffino, Lorenzo
Benati, Donatella
Malatesta, Manuela
Sbarbati, Andrea
Marzola, Pasquina
Mariotti, Raffaella
2017
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Busato, Alice
Bonafede, Roberta
Bontempi, Pietro
Scambi, Ilaria
Schiaffino, Lorenzo
Benati, Donatella
Malatesta, Manuela
Sbarbati, Andrea
Marzola, Pasquina
Mariotti, Raffaella
Titel
Labeling and Magnetic Resonance Imaging of Exosomes Isolated from Adipose Stem Cells
Ist Teil von
Current protocols in cell biology, 2017-06, Vol.75 (1), p.3.44.1-3.44.15
Ort / Verlag
United States
Erscheinungsjahr
2017
Quelle
MEDLINE
Beschreibungen/Notizen
Adipose stem cells (ASC) represent a promising therapeutic approach for neurodegenerative diseases. Most biological effects of ASC are probably mediated by extracellular vesicles, such as exosomes, which influence the surrounding cells. Current development of exosome therapies requires efficient and noninvasive methods to localize, monitor, and track the exosomes. Among imaging methods used for this purpose, magnetic resonance imaging (MRI) has advantages: high spatial resolution, rapid in vivo acquisition, and radiation‐free operation. To be detectable with MRI, exosomes must be labeled with MR contrast agents, such as ultra‐small superparamagnetic iron oxide nanoparticles (USPIO). Here, we set up an innovative approach for exosome labeling that preserves their morphology and physiological characteristics. We show that by labeling ASC with USPIO before extraction of nanovesicles, the isolated exosomes retain nanoparticles and can be visualized by MRI. The current work aims at validating this novel USPIO‐based exosome labeling method by monitoring the efficiency of the labeling with MRI both in ASC and in exosomes. © 2017 by John Wiley & Sons, Inc.
Sprache
Englisch
Identifikatoren
ISSN: 1934-2500
eISSN: 1934-2616
DOI: 10.1002/cpcb.23
Titel-ID: cdi_proquest_miscellaneous_1911209260
Format
–
Schlagworte
Adipose Tissue - cytology
,
Animals
,
Cells, Cultured
,
Contrast Media - chemistry
,
Dextrans - chemistry
,
exosome labeling
,
Exosomes - chemistry
,
Magnetic Resonance Imaging - methods
,
Magnetite Nanoparticles - chemistry
,
Mice, Inbred C57BL
,
MRI
,
Staining and Labeling - methods
,
Stem Cells - chemistry
,
Stem Cells - cytology
,
stem cells labeling
,
superparamagnetic iron oxide nanoparticles
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