Details

Autor(en) / Beteiligte

• Fernandes, K.R.
• Magri, A.M.P.
• Kido, H.W.
• Parisi, J.R.
• Assis, L.
• Fernandes, K.P.S.
• Mesquita-Ferrari, R.A.
• Martins, V.C.
• Plepis, A.M.
• Zanotto, E.D.
• Peitl, O.
• Renno, A.C.M.

Titel

Biosilicate/PLGA osteogenic effects modulated by laser therapy: In vitro and in vivo studies

Ist Teil von

• Journal of photochemistry and photobiology. B, Biology, 2017-08, Vol.173, p.258-265

Ort / Verlag

Switzerland: Elsevier B.V

Erscheinungsjahr

2017

Quelle

Elsevier ScienceDirect Journals

Beschreibungen/Notizen

• The main purpose of the present work was to evaluate if low laser level therapy (LLLT) can improve the effects of Biosilicate®/PLGA (BS/PLGA) composites on cell viability and bone consolidation using a tibial defects of rats. The composites were characterized by scanning electron microscope (SEM) and reflection Fourier transform infrared spectrometer (FTIR). For the in vitro study, fibroblast and osteoblast cells were seeded in the extract of the composites irradiated or not with LLLT (Ga-Al-As, 808nm, 10J/cm2) to assess cell viability after 24, 48 and 72h. For the in vivo study, 80 Wistar rats with tibial bone defects were distributed into 4 groups (BS; BS+LLLT; BS/PLGA and BS/PLGA+LLLT) and euthanized after 2 and 6weeks. Laser irradiation Ga-Al-As (808nm, 30J/cm2) in the rats was performed 3 times a week. The SEM and FTIR results revealed that PLGA were successfully inserted into BS and the microparticles degraded over time. The in vitro findings demonstrated higher fibroblast viability in both BS/PLGA groups after 24h and higher osteoblast viability in BS/PLGA+LLLT in all periods. As a conclusion, animals treated with BS/PLGA+LLLT demonstrated an improved material degradation and an increased amount of granulation tissue and newly formed bone. •The microparticles of PLGA inserted into BS degraded over time, leaving pores into the material.•Biosilicate®/PLGA composites irradiated with LLLT improve the osteoblasts viability.•Biosilicate®/PLGA associated to LLLT were effective in stimulating bone formation in an experimental model of tibial defect.