Details

Autor(en) / Beteiligte

• de Sain-van der Velden, Monique G.M.
• van der Ham, Maria
• Gerrits, Johan
• Prinsen, Hubertus C.M.T.
• Willemsen, Marcel
• Pras-Raves, Mia L.
• Jans, Judith J.
• Verhoeven-Duif, Nanda M.

Titel

Quantification of metabolites in dried blood spots by direct infusion high resolution mass spectrometry

Ist Teil von

• Analytica chimica acta, 2017-08, Vol.979, p.45-50

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• Diagnosis and treatment of inborn errors of metabolism (IEM) require the analysis of a variety of metabolites. These compounds are usually quantified by targeted platforms. High resolution mass spectrometry (HRMS) has the potential to detect hundreds to thousands of metabolites simultaneously. A chip-based nanoelectrospray source (chip-based nanoESI) enables the direct infusion of biological samples. Major advantages of this system include high sample throughput, no sample carryover, and low sample consumption. The combination, chip-based nanoESI-HRMS enables untargeted metabolomics of biological samples but its potential for quantification of metabolites has not been reported. We investigated whether chip-based nanoESI-HRMS is suitable for quantification of metabolites in dried blood spots (DBS). After addition of internal standards, metabolites were extracted with methanol. Aliquots of each extract were analysed by chip-based nanoESI-HRMS operating in both positive and negative mode with an m/z window of 70–600 and a resolution of 140,000. Total run time was 4.5 min per sample and a full report could be generated within 40 min. Concentrations of all 21 investigated diagnostic metabolites in DBS as quantified by chip-based nanoESI-HRMS correlated well with those obtained by targeted liquid chromatography-tandem mass spectrometry. We conclude that chip-based nanoESI-HRMS is suitable for quantification. •Separate analyses of metabolite groups are often needed for optimally assessing the patients metabolic status.•Quantification of these different markers has a long turnaround time.•Chip-based nanoESI HRMS is a good alternative for current methods.•CChip-based nanoESI HRMS is extremely fast (with analytical run times of 4.5 min per sample).•Chip-based nanoESI HRMS is very efficient (many different markers in one run).