Details

Autor(en) / Beteiligte

• Rodriguez, Maria J.
• Mascaraque, Ainhoa
• Ramos‐Soriano, Javier
• Torres, Maria J.
• Perkins, James R.
• Gomez, Francisca
• Garrido‐Arandia, Maria
• Cubells‐Baeza, Nuria
• Andreu, David
• Diaz‐Perales, Araceli
• Rojo, Javier
• Mayorga, Cristobalina

Titel

Pru p 3‐Epitope‐based sublingual immunotherapy in a murine model for the treatment of peach allergy

Ist Teil von

• Molecular nutrition & food research, 2017-10, Vol.61 (10), p.n/a

Ort / Verlag

Germany: Wiley Subscription Services, Inc

Erscheinungsjahr

2017

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Scope Food‐specific immunotherapy (SIT) is a promising treatment for lipid transfer protein (LTP)‐syndrome. We propose a novel sublingual‐SIT (SLIT) that combines a Pru p 3 T‐cell peptide and an oligodeoxyribonucleotide (ODN) with CpG motifs (ODN‐CpG) as adjuvants to induce a specific Th1/Treg response. Methods and results LTP‐peach allergic mice were treated sublingually with a combination of a CpG sequence and mono‐ or tetravalent systems including a Pru p 3 peptide, D1(Prup3) or D4(Prup3). Mice were challenged intraperitoneally with Pru p 3 one or three weeks after SLIT and tolerance was assessed. Mice treated with D1(Prup3)+CpG were protected from anaphylaxis after Pru p 3 challenge. They showed no change in body temperature, lower levels of Pru p 3‐specific IgE and IgG1 antibodies and higher levels of sIgG2a compared to the untreated group. They had fewer IgE and IgG1 secreting cells and more sIgG2a secreting cells. Moreover, a significantly lower number of Pru p 3‐specific CD4+T cells and a higher number of Treg cells were found, alongside a Th1 cytokine pattern. These changes were maintained for three weeks after stopping treatment. Conclusion D1Prup3+CpG represents a promising SIT for food allergy. It is easily synthesized and induces protection from anaphylaxis to Pru p 3 that is maintained for at least three weeks. In this study, LTP‐peach anaphylactic mice are treated sublingually with a combination of a CpG sequence and mono‐ or tetravalent systems including a Pru p 3 peptide. It is found that the monomeric compound administered sublingually represents a promising specific immunotherapy for food allergy since it is easily synthesized, safe, and induces a persistent protection from anaphylaxis to Pru p 3.