International immunopharmacology, 2017-08, Vol.49, p.142-147
2017
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- Autor(en) / Beteiligte
- Titel
- Schisandrin B inhibits LPS-induced inflammatory response in human umbilical vein endothelial cells by activating Nrf2
- Ist Teil von
- International immunopharmacology, 2017-08, Vol.49, p.142-147
- Ort / Verlag
- Netherlands: Elsevier B.V
- Erscheinungsjahr
- 2017
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Schisandrin B (SchB), an active ingredient extracted from Schisandra chinensis (Turcz.) Baill, has been known to have anti-oxidant and anti-inflammatory activities. In this study, we investigated the anti-inflammatory effects and mechanism of SchB in LPS-stimulated human umbilical vein endothelial cells (HUVECs). The effects of SchB on VCAM-1, ICAM-1, NF-κB and Nrf2 expression were detected by western blot analysis. The effects of SchB on TNF-α and IL-8 production were detected by ELISA. The results showed that SchB strongly suppressed the production of TNF-α and IL-8 in HUVECs stimulated with LPS. SchB also inhibited LPS-induced VCAM-1 and ICAM-1 expression. Furthermore, SchB blocked the activation of NF-κB induced by LPS. In addition, SchB increased the expression of Nrf2 and HO-1 in a concentration-dependent manner. And the inhibition of TNF-α and IL-8 production by SchB was blocked by transfection with Nrf2 siRNA. Our findings showed that SchB inhibited LPS-induced inflammation in HUVECs by activating Nrf2 signaling pathway. •SchB inhibited the production of TNF-α and IL-8 in HUVECs stimulated with LPS.•SchB also inhibited LPS-induced VCAM-1 and ICAM-1 expression.•SchB blocked the activation of NF-κB induced by LPS.•SchB increased the expression of Nrf2 and HO-1 in a concentration-dependent manner.•The inhibition of TNF-α and IL-8 production by SchB was blocked by transfection with Nrf2 siRNA.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1567-5769eISSN: 1878-1705DOI: 10.1016/j.intimp.2017.05.032Titel-ID: cdi_proquest_miscellaneous_1906138054
- Format
- –
- Schlagworte
- Anti-Inflammatory Agents - pharmacology, Berries, Chemical compounds, Cyclooctanes - pharmacology, Cytokine, Cytokines, Endothelial cells, Endothelium, Endothelium, Vascular - drug effects, Endothelium, Vascular - immunology, Enzyme-linked immunosorbent assay, Heme Oxygenase-1 - metabolism, Human behavior, Human Umbilical Vein Endothelial Cells, Humans, Inflammation, Inflammation - drug therapy, Inflammatory response, Intercellular adhesion molecule 1, Intercellular Adhesion Molecule-1 - metabolism, Interleukin 8, Interleukin-8 - metabolism, Lignans - pharmacology, Lipopolysaccharides, Lipopolysaccharides - immunology, NF-E2-Related Factor 2 - genetics, NF-E2-Related Factor 2 - metabolism, NF-kappa B - metabolism, NF-κB, Nrf2, Polycyclic Compounds - pharmacology, RNA, Small Interfering - genetics, Schisandra - immunology, Schisandrin B, Signal Transduction, siRNA, Transfection, Tumor necrosis factor, Tumor Necrosis Factor-alpha - metabolism, Umbilical vein, Vascular cell adhesion molecule 1, Vascular Cell Adhesion Molecule-1 - metabolism, Veins & arteries