Details

Autor(en) / Beteiligte

• Bayer, Andreas
• Lammel, Justus
• Lippross, Sebastian
• Klüter, Tim
• Behrendt, Peter
• Tohidnezhad, Mersedeh
• Pufe, Thomas
• Cremer, Jochen
• Jahr, Holger
• Rademacher, Franziska
• Gläser, Regine
• Harder, Jürgen

Titel

Platelet-released growth factors induce psoriasin in keratinocytes: Implications for the cutaneous barrier

Ist Teil von

• Annals of anatomy, 2017-09, Vol.213, p.25-32

Ort / Verlag

Germany: Elsevier GmbH

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• Millions of patients around the world suffer minor or major extremity amputation due to progressive wound healing complications of chronic or infected wounds, the therapy of which remains a challenge. One emerging therapeutic option for the treatment of these complicated wounds is the local application of an autologous thrombocytes concentrate lysate (e.g. platelet-released growth factors ((PRGF)) or Vivostat PRF®) that contains a multitude of chemokines, cytokines and growth factors and is therefore supposed to stimulate the complex wound healing process. Although PRGF and Vivostat PRF® are already used successfully to support healing of chronic, hard-to-heal and infected wounds the underlying molecular mechanisms are not well understood. Psoriasin, also termed S100A7, is a multifunctional antimicrobial protein expressed in keratinocytes and is involved in various processes such as wound-healing, angiogenesis, innate immunity and immune-modulation. In this study, we investigated the influence of PRGF on psoriasin expression in human primary keratinocytes in vitro and the influence of Vivostat PRF® on psoriasin expression in experimentally generated skin wounds in vivo. PRGF treatment of primary keratinocytes caused a significant concentration- and time-dependent increase of psoriasin gene and protein expression in vitro that were partially mediated by the epidermal growth factor receptor (EGFR) and the interleukin-6 receptor (IL-6R). In accordance with these cell culture data, Vivostat PRF® induced a significant psoriasin gene and protein expression when applied to artificially generated skin wounds in vivo. The observed psoriasin induction in keratinocytes may contribute to the wound healing-promoting effects of therapeutically used thrombocyte concentrate lysates.