Details

Autor(en) / Beteiligte

• Wu, Zhong-Ming
• Wang, Li
• Zhu, Wei
• Gao, Ying-Hua
• Wu, Hai-Ming
• Wang, Mi
• Hu, Tai-Shan
• Yan, Yi-Jia
• Chen, Zhi-Long

Titel

Preparation of a chlorophyll derivative and investigation of its photodynamic activities against cholangiocarcinoma

Ist Teil von

• Biomedicine & pharmacotherapy, 2017-08, Vol.92, p.285-292

Ort / Verlag

France: Elsevier Masson SAS

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• Abstract Photodynamic therapy (PDT) is emerging as a promising method for the treatment of various cancer diseases. However, the clinical application of PDT is limited due to the lack of effective photosensitizers. In this study, a novel chlorophyll derivative, N,N -bis(2-carboxyethyl)pyropheophorbide a (BPPA), had been synthesized and characterized. BPPA had a characteristic long wavelength absorption peak at 669 nm and a singlet oxygen quantum yield of 0.54. To investigate the photodynamic ability of BPPA against cholangiocarcinoma (CCA), cellular uptake, subcellular location and bio-distribution, in vitro and in vivo PDT efficacy of BPPA were studied. The results showed that BPPA could rapidly accumulate in QBC-939 cells and localize in the cytoplasm. BPPA- PDT was effective in reducing the cell viability in a drug dose- and light dose-dependent manner in vitro . In CCA xenograft nude mouse model, the concentration of BPPA in the plasma lowered rapidly, and the fluorescence signal peaked at 0.5 h and 2 h after injection in the skin and tumor, respectively. Significant quantities could be observed in the tumor. BPPA followed by irradiation could significantly inhibit growth of tumors, and histological examination revealed necrotic damage in PDT-treated tumors. These results suggested that BPPA could be a promising drug candidate for photodynamic therapy in cholangiocarcinoma.