Details

Autor(en) / Beteiligte

• Han, Guobin
• Zeng, Qiaoling
• Jiang, Zhongwei
• Deng, Wenchan
• Huang, Chengzhi
• Li, Yuanfang

Titel

Simple preparation of magnetic metal-organic frameworks composite as a “bait” for phosphoproteome research

Ist Teil von

• Talanta (Oxford), 2017-08, Vol.171, p.283-290

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2017

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Phosphospecific enrichment techniques and mass spectrometry (MS) are primary tools for comprehending the cellular phosphoproteome. In this work, a rational and extremely facile route to synthesize the magnetic metal-organic frameworks (mMOFs) was employed and the prepared composite was first utilized as a “bait” for selective enrichment of phosphopeptides. Typically, the mMOFs was synthesized via electrostatic self-assembly between the negatively charged Fe3O4 magnetic nanoparticles (MNPs) and positively charged MIL-101(Fe). The obtained Fe3O4/MIL-101(Fe) composite possessed well-defined structures, rough surface, highly specific surface area and excellent magnetic property. To demonstrate their ability for enrichment of phosphopeptides, we applied Fe3O4/MIL-101(Fe) as a “bait” to capture the phosphopeptides from standard protein digestion and practical samples. The enriched phosphopeptides were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The MS results show that the Fe3O4/MIL-101(Fe) exhibits superior enrichment performance for phosphopeptides with low detectable concentration assessed to be 8 fmol, selectivity investigated to be 1:1000 using β-casein/bovine serum albumin mixture and enrichment recovery evaluated to be 89.8%. Based on these excellent properties, the prepared composite was used to enrich the phosphopeptides from tilapia eggs biological samples for the first time. A total number of 51 phosphorylation sites were identified from the digest of tilapia eggs proteins, suggesting the excellent potential of Fe3O4/MIL-101(Fe) composite in the practical application. A one-step operation via electrostatic self-assembly was employed to synthesize the Fe3O4/MIL-101(Fe) composite and the as-prepared composite demonstrated a powerful application for efficiently selective enrichment of phosphopeptides. Compared with many IMAC and MOAC nanomaterials reported previously, the Fe3O4/MIL-101(Fe) displayed excellent sensitivity, admirable selectivity and superior recovery. Furthermore, the remarkable results obtained from tryptic digest of tilapia eggs samples suggested that the Fe3O4/MIL-101(Fe) composite could exhibit excellent potential in the practical application. [Display omitted] •A rational and facile route to synthesize the mMOFs composite is employed.•This composite displayed superior selectivity and recovery for phosphopeptides.•The phosphopeptides from tilapia eggs were identified for the first time.