Details

Autor(en) / Beteiligte

• Janson, David
• Rietveld, Marion
• Mahé, Christian
• Saintigny, Gaëlle
• El Ghalbzouri, Abdoelwaheb

Titel

Differential effect of extracellular matrix derived from papillary and reticular fibroblasts on epidermal development in vitro

Ist Teil von

• EJD. European journal of dermatology, 2017-05, Vol.27 (3), p.237-246

Ort / Verlag

Paris: John Libbey Eurotext

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• Background Papillary and reticular fibroblasts have different effects on keratinocyte proliferation and differentiation. Objectives The aim of this study was to investigate whether these effects are caused by differential secretion of soluble factors or by differential generation of extracellular matrix from papillary and reticular fibroblasts. Materials & Methods To study the effect of soluble factors, keratinocyte monolayer cultures were grown in papillary or reticular fibroblast-conditioned medium. To study the effect of extracellular matrix, keratinocytes were grown on papillary or reticular-derived matrix. Results Conditioned medium from papillary or reticular fibroblasts did not differentially affect keratinocyte viability or epidermal development. However, keratinocyte viability was increased when grown on matrix derived from papillary, compared with reticular, fibroblasts. In addition, the longevity of the epidermis was increased when cultured on papillary fibroblast-derived matrix skin equivalents compared with reticular-derived matrix skin equivalents. Conclusion The findings indicate that the matrix secreted by papillary and reticular fibroblasts is the main causal factor to account for the differences in keratinocyte growth and viability observed in our study. Differences in response to soluble factors between both populations were less significant. Matrix components specific to the papillary dermis may account for the preferential growth of keratinocytes on papillary dermis.