Details

Autor(en) / Beteiligte

• Dong, Xiaoyun
• Jin, Yingli
• Ming, Di
• Li, Bangbang
• Dong, Haisi
• Wang, Lin
• Wang, Tiedong
• Wang, Dacheng

Titel

CRISPR/dCas9-mediated inhibition of gene expression in Staphylococcus aureus

Ist Teil von

• Journal of microbiological methods, 2017-08, Vol.139, p.79-86

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• The understanding of the genetic mechanism of Staphylococcus aureus requires efficient tools, however, genetic manipulation in S. aureus is always laborious and time-consuming. Here we proposed a novel CRISPR/dCas9 interference method for the rapid knockdown of target genes. Furthermore, multiple genes can be repressed simultaneously by using this method. •A novel method for inhibition of gene expression in Staphylococcus aureus using CRISPR/dCas9•The method is suitable for rapid knockdown gene expression.•The method is verified for repressing multiple genes simultaneously.