Bioscience, biotechnology, and biochemistry, 2017-06, Vol.81 (6), p.1136-1147
2017
Details
- Autor(en) / Beteiligte
- Titel
- The distribution of vinculin to lipid rafts plays an important role in sensing stiffness of extracellular matrix
- Ist Teil von
- Bioscience, biotechnology, and biochemistry, 2017-06, Vol.81 (6), p.1136-1147
- Ort / Verlag
- England: Taylor & Francis
- Erscheinungsjahr
- 2017
- Link zum Volltext
- Quelle
- Taylor & Francis Journals Auto-Holdings Collection
- Beschreibungen/Notizen
- Extracellular matrix (ECM) stiffness regulates cell differentiation, survival, and migration. Our previous study has shown that the interaction of the focal adhesion protein vinculin with vinexin α plays a critical role in sensing ECM stiffness and regulating stiffness-dependent cell migration. However, the mechanism how vinculin-vinexin α interaction affects stiffness-dependent cell migration is unclear. Lipid rafts are membrane microdomains that are known to affect ECM-induced signals and cell behaviors. Here, we show that vinculin and vinexin α can localize to lipid rafts. Cell-ECM adhesion, intracellular tension, and a rigid ECM promote vinculin distribution to lipid rafts. The disruption of lipid rafts with Methyl-β-cyclodextrin impaired the ECM stiffness-mediated regulation of vinculin behavior and rapid cell migration on rigid ECM. These results indicate that lipid rafts play an important role in ECM-stiffness regulation of cell migration via vinculin. Vinculin is in non-raft on soft ECM. Rigid ECM promotes vinculin binding to vinexin α and PIP 2 , leading to distribution to raft and enhanced migration
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0916-8451eISSN: 1347-6947DOI: 10.1080/09168451.2017.1289074Titel-ID: cdi_proquest_miscellaneous_1896892485
- Format
- –
- Schlagworte
- Animals, beta-Cyclodextrins - pharmacology, Biomechanical Phenomena, Caveolin 1 - genetics, Caveolin 1 - metabolism, Cell Adhesion - drug effects, Cell Line, Cell Movement - drug effects, Embryo, Mammalian, extracellular matrix, Extracellular Matrix - drug effects, Extracellular Matrix - metabolism, Extracellular Matrix - ultrastructure, Fibroblasts - drug effects, Fibroblasts - metabolism, Fibroblasts - ultrastructure, focal adhesions, Focal Adhesions - drug effects, Focal Adhesions - metabolism, Focal Adhesions - ultrastructure, Gene Expression Regulation, Hardness, lipid raft, mechanotransduction, Membrane Microdomains - drug effects, Membrane Microdomains - metabolism, Membrane Microdomains - ultrastructure, Membrane Proteins - genetics, Membrane Proteins - metabolism, Mice, Muscle Proteins - genetics, Muscle Proteins - metabolism, Paxillin - genetics, Paxillin - metabolism, Protein Binding, Protein Isoforms - genetics, Protein Isoforms - metabolism, Signal Transduction, vinculin, Vinculin - genetics, Vinculin - metabolism