Details

Autor(en) / Beteiligte

• Higuchi, Akon
• Higuchi, Yuichiro
• Furuta, Kentaro
• Yoon, Boo Ok
• Hara, Mariko
• Maniwa, Syunji
• Saitoh, Masatoshi
• Sanui, Kohei

Titel

Chiral separation of phenylalanine by ultrafiltration through immobilized DNA membranes

Ist Teil von

• Journal of membrane science, 2003-08, Vol.221 (1), p.207-218

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

2003

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• In the current studies, we investigated the use of immobilized DNA membranes for chiral separation. d-Phenylalanine preferentially permeated through immobilized DNA membranes with a pore size <2.0 nm (molecular weight cut-off (MWCO)<5000) while l-phenylalanine preferentially permeated through immobilized DNA membranes with a pore size >2.0 nm (MWCO>5000). The pore size of the immobilized DNA membranes regulated preferential permeation of the enantiomer through the membranes. The immobilized DNA membranes are categorized as channel-type membranes and not as affinity membranes. We further propose a multi-stage cascade for chiral separation by ultrafiltration. When the two types of the membranes were used in a cascade filtration system, there was no change of concentration ratio between l-enantiomer and d-enantiomer in the feed solution at each stage. Only two membranes were necessary in a multi-stage processes if both membrane types were used.