Details

Autor(en) / Beteiligte

• Gonzalez, Miguel A
• Barriga, Hanna M G
• Richens, Joanna L
• Law, Robert V
• O'Shea, Paul
• Bresme, Fernando

Titel

How does ytterbium chloride interact with DMPC bilayers? A computational and experimental study

Ist Teil von

• Physical chemistry chemical physics : PCCP, 2017, Vol.19 (13), p.9199-9209

Ort / Verlag

England

Erscheinungsjahr

2017

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Lanthanide salts have been studied for many years, primarily in Nuclear Magnetic Resonance (NMR) experiments of mixed lipid-protein systems and more recently to study lipid flip-flop in model membrane systems. It is well recognised that lanthanide salts can influence the behaviour of both lipid and protein systems, however a full molecular level description of lipid-lanthanide interactions is still outstanding. Here we present a study of lanthanide-bilayer interactions, using molecular dynamics computer simulations, fluorescence electrostatic potential experiments and nuclear magnetic resonance. Computer simulations reveal the microscopic structure of DMPC lipid bilayers in the presence of Yb , and a surprising ability of the membranes to adsorb significant concentrations of Yb without disrupting the overall membrane structure. At concentrations commonly used in NMR experiments, Yb ions bind strongly to 5 lipids, inducing a small decrease of the area per lipid and a slight increase of the ordering of the aliphatic chains and the bilayer thickness. The area compressibility modulus increases by a factor of two, with respect to the free-salt case, showing that Yb ions make the bilayer more rigid. These modifications of the bilayer properties should be taken into account in the interpretation of NMR experiments.