Details

Autor(en) / Beteiligte

• Chen, Chao
• Van der Borght, Jef
• De Vreese, Rob
• D'hooghe, Matthias
• Soetaert, Wim
• Desmet, Tom

Titel

Engineering the specificity of trehalose phosphorylase as a general strategy for the production of glycosyl phosphates

Ist Teil von

• Chemical communications (Cambridge, England), 2014-07, Vol.50 (58), p.7834-7836

Ort / Verlag

England

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• A two-step process is reported for the anomeric phosphorylation of galactose, using trehalose phosphorylase as biocatalyst. The monosaccharide enters this process as acceptor but can subsequently be released from the donor side, thanks to the non-reducing nature of the disaccharide intermediate. A key development was the creation of an optimized enzyme variant that displays a strict specificity (99%) for β-galactose 1-phosphate as product.