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Engineering the specificity of trehalose phosphorylase as a general strategy for the production of glycosyl phosphates
Ist Teil von
Chemical communications (Cambridge, England), 2014-07, Vol.50 (58), p.7834-7836
Ort / Verlag
England
Erscheinungsjahr
2014
Quelle
MEDLINE
Beschreibungen/Notizen
A two-step process is reported for the anomeric phosphorylation of galactose, using trehalose phosphorylase as biocatalyst. The monosaccharide enters this process as acceptor but can subsequently be released from the donor side, thanks to the non-reducing nature of the disaccharide intermediate. A key development was the creation of an optimized enzyme variant that displays a strict specificity (99%) for β-galactose 1-phosphate as product.