Details

Autor(en) / Beteiligte

• Reiter, Jill L
• Drendel, Holli M
• Chakraborty, Sujata
• Schellinger, Megan M
• Lee, Men-Jean
• Mor, Gil

Titel

Cytogenetic features of human trophoblast cell lines SWAN-71 and 3A-subE

Ist Teil von

• Placenta (Eastbourne), 2017-04, Vol.52, p.17-20

Ort / Verlag

Netherlands: Elsevier Ltd

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• Abstract Immortalization of primary cells with telomerase is thought to maintain normal phenotypic properties and avoid chromosomal abnormalities and other cancer-associated changes that occur following simian virus 40 tumor antigen (SV40 Tag) induced immortalization. However, we report that the human telomerase reverse transcriptase (hTERT)-immortalized SWAN-71 trophoblast cell line has a near pentaploid 103∼119,XXXX[cp20] karyotype. Additionally, DNA typing analysis indicated that SWAN-71 cells have acquired microsatellite instability. In comparison, the post-crisis SV40-transformed trophoblast cell line 3A-subE was hypertriploid 69∼81,XX[cp20]. Both cell lines contained multiple specific clonal rearrangements. These findings emphasize the need to monitor for genetic instability in hTERT-immortalized cells.