Details

Autor(en) / Beteiligte

• Ponce Barraza, Elizama
• Cárdenas Reyna, Tomás
• Angulo, Carlos
• Herrera Ramírez, José Carlomán
• López, Gilberto V.
• Medina-Basulto, Gerardo Enrique
• Eda, Shigetoshi
• Hori-Oshima, Sawako

Titel

Evaluation of two in-house immunoenzymatic tests to serodiagnose subclinical paratuberculisis in a sheep flock in Mexicali valley, Mexico

Ist Teil von

• Journal of immunoassay & immunochemistry, 2017-07, Vol.38 (4), p.420-429

Ort / Verlag

England: Taylor & Francis

Erscheinungsjahr

2017

Quelle

Taylor & Francis

Beschreibungen/Notizen

• Paratuberculosis (PTB) or Johne's disease is a common ruminant infectious disease caused by Mycobacterium avium subsp. paratuberculosis (MAP). In this study, two MAP antigens were compared for their diagnostic utility to detect subclinical PTB in a sheep flock in Mexicali, Mexico. Sheep (n = 31) without clinical signs but positive on a direct fecal-polymerase chain reaction were tested with two preabsorbed in-house enzyme linked immunosorbent assays (ELISAs) using: (1) an ethanol-extracted surface lipid antigen (EVELISA) and (2) a protoplasmic antigen (ELISA-PPA). Sensitivities of the EVELISA and ELISA-PPA were 84% (95% CI; 66-95%) and 29% (95% CI; 14-48%), respectively. The EVELISA test could be a fast and effective way to identify subclinical ovine PTB for severely affected flocks.