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Flow cytometric determination of lipid content in a marine dinoflagellate, Crypthecodinium cohnii
Ist Teil von
Journal of applied phycology, 2003-09, Vol.15 (5), p.433-438
Ort / Verlag
Dordrecht: Springer
Erscheinungsjahr
2003
Link zum Volltext
Quelle
SpringerLink
Beschreibungen/Notizen
Since flow cytometry allows rapid, simultaneous and quantitative measurements related to cell morphology and physiologicy, the lipid-specific fluorescent dye, Nile Red, was employed for the in vivo lipid quantification of Crypthecodinium cohnii, a heterotrophic marine dinoflagellate rich in polyunsaturated long chain fatty acids. The fluorescence signal was linearly correlated with the neutral and polar lipid content as determined by gravimetric techniques. A significant correlation of NR signal was also observed between the polar to neutral lipid ratio and docohexaenoic acid per cell. The results demonstrate a method for rapid lipid quantification that can be used in the selection, isolation and culture control of C. cohnii clones with high lipid and DHA content.