Journal of diabetes and its complications, 2017-01, Vol.31 (1), p.241-244
2017
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- Autor(en) / Beteiligte
- Titel
- Increasing the miR-126 expression in the peripheral blood of patients with diabetic foot ulcers treated with maggot debridement therapy
- Ist Teil von
- Journal of diabetes and its complications, 2017-01, Vol.31 (1), p.241-244
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2017
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Abstract Background miR-126 may increase angiogenesis in patients with diabetic foot ulcers (DFUs) treated with maggot debridement therapy (MDT). Methods Real-time quantitative PCR was used to detect expression of miR-126 mRNA in the peripheral blood among the non-diabetic population, type 2 diabetes mellitus patients without DFU, and patients with DFUs of type 2 diabetes mellitus. The expression of miR-126 mRNA in the peripheral blood of patients with DFUs was observed before and after MDT. Finally, human umbilical vein endothelial cells (HUVEC) were utilized to explore miR-126 mRNA expression with maggot excretions/secretions (ES). Results In the patients with DFUs, the miR-126 mRNA expression level in the peripheral blood was less than that type 2 diabetes mellitus patients without DFU, and much lower than that in the non-diabetic population (P < 0.001). The miR-126 expression level was significantly increased in those DFU patients treated with MDT (P < 0.05). Finally, using HUVEC co-cultured with ES, we showed the ES increased miR-126 expression in vitro (P < 0.001). Conclusion MDT upregulates the miR-126 expression in the peripheral blood of patients with DFUs.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1056-8727eISSN: 1873-460XDOI: 10.1016/j.jdiacomp.2016.07.026Titel-ID: cdi_proquest_miscellaneous_1885868388
- Format
- –
- Schlagworte
- Aged, Angiogenesis, Animals, Apoptosis, Biological Therapy - methods, Bodily Secretions - physiology, Cardiovascular disease, Cell adhesion & migration, Cell growth, Cells, Cultured, China, Coculture Techniques, Complementary Therapies, Debridement - methods, Diabetes, Diabetes Mellitus, Type 2 - complications, Diabetic Foot - blood, Diabetic Foot - metabolism, Diabetic Foot - pathology, Diabetic Foot - therapy, Diabetic foot ulcer, Diptera - physiology, Endocrinology & Metabolism, Endothelium, Vascular - metabolism, Endothelium, Vascular - pathology, Expression, Female, Foot diseases, Gene expression, Gene Expression Regulation, Germ-Free Life, Hospitals, Urban, Human Umbilical Vein Endothelial Cells - cytology, Human Umbilical Vein Endothelial Cells - metabolism, Human Umbilical Vein Endothelial Cells - pathology, Humans, Laboratories, Larva - physiology, Leg ulcers, Maggot debridement therapy, Male, MicroRNAs - agonists, MicroRNAs - blood, MicroRNAs - metabolism, Middle Aged, miR-126, miRNA, Plasma, Up-Regulation, Vascular endothelial growth factor, Wound healing