Details

Autor(en) / Beteiligte

• Marques, M P M
• Batista de Carvalho, A L M
• Sakai, V Garcia
• Hatter, L
• Batista de Carvalho, L A E

Titel

Intracellular water - an overlooked drug target? Cisplatin impact in cancer cells probed by neutrons

Ist Teil von

• Physical chemistry chemical physics : PCCP, 2017, Vol.19 (4), p.2702-2713

Ort / Verlag

England

Erscheinungsjahr

2017

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• The first neutron scattering study on human nucleated cells is reported, addressing the subject of solvent-slaving to a drug by probing intracellular water upon drug exposure. Inelastic and quasi-elastic neutron scattering spectroscopy with isotope labelling was applied for monitoring interfacial water response to the anticancer drug cisplatin, in the low prognosis human metastatic breast cancer cells MDA-MB-231. Optical vibrational data were also obtained for lyophilised cells. Concentration-dependent dynamical changes evidencing a progressive mobility reduction were unveiled between untreated and cisplatin-exposed samples, concurrent with variations in the native organisation of water molecules within the intracellular medium as a consequence of drug action. The results thus obtained yielded a clear picture of the intracellular water response to cisplatin and constitute the first reported experimental proof of a drug impact on the cytomatrix by neutron techniques. This is an innovative way of tackling a drug's pharmacodynamics, searching for alternative targets of drug action.