Details

Autor(en) / Beteiligte

• Giarmarco, Michelle M
• Cleghorn, Whitney M
• Sloat, Stephanie R
• Hurley, James B
• Brockerhoff, Susan E

Titel

Mitochondria Maintain Distinct Ca super(2+) Pools in Cone Photoreceptors

Ist Teil von

• The Journal of neuroscience, 2017-02, Vol.37 (8), p.2061-2072

Erscheinungsjahr

2017

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Ca super(2+) ions have distinct roles in the outer segment, cell body, and synaptic terminal of photoreceptors. We tested the hypothesis that distinct Ca super(2+) domains are maintained by Ca super(2+) uptake into mitochondria. Serial block face scanning electron microscopy of zebrafish cones revealed that nearly 100 mitochondria cluster at the apical side of the inner segment, directly below the outer segment. The endoplasmic reticulum surrounds the basal and lateral surfaces of this cluster, but does not reach the apical surface or penetrate into the cluster. Using genetically encoded Ca super(2+) sensors, we found that mitochondria take up Ca super(2+) when it accumulates either in the cone cell body or outer segment. Blocking mitochondrial Ca super(2+) uniporter activity compromises the ability of mitochondria to maintain distinct Ca super(2+) domains. Together, our findings indicate that mitochondria can modulate subcellular functional specialization in photoreceptors.