Details

Autor(en) / Beteiligte

• Da Silva, Wander José
• Gonçalves, Letícia Machado
• Viu, Flávia Carvalho
• Leal, Cristiane Maria Brasil
• Barbosa, Célia Marisa Rizatti
• Del Bel Cury, Altair Antoninha

Titel

Surface roughness influences Candida albicans biofilm formation on denture materials

Ist Teil von

• Revista odonto ciência, 2016, Vol.31 (2), p.54

Ort / Verlag

Porto Alegre: EDIPUCRS

Erscheinungsjahr

2016

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Abstract Objective: This study aimed to evaluate the influences of surface roughness (SR) of denture base and liner materials on Candida albicans biofilm formation. Methods: Discs were fabricated using poly (methyl methacrylate) acrylic resin and poly(ethyl methacrylate) denture liner, according to manufacturers' instructions. Both acrylic resin and relined discs were finished or finished + polished and the SR was measured. Discs were saliva-coated, and C. albicans biofilms were developed on such surfaces for 48 hours. Biofilms were evaluated for cell counts, metabolic activity, and structural characteristics. Data were analyzed by one-way ANOVA and Tukey tests using a significance level of 5%. Results: Finished acrylic resin and denture liner discs had higher SR compared to finished + polished discs (P<0.001). Finished + polished acrylic resin discs contained fewer cells compared to only finished discs (P<0.001). No metabolic activity differences were seen between biofilms formed on both groups (P>0.05). Bulky, thick and less rough biofilms were formed on only finished denture materials (P<0.05). Confocal images reveal increased presence of black spaces for biofilms developed on finished + polished discs. Conclusion: Reduced SR resulted in decreased C. albicans biofilm accumulation on both denture materials.