Iranian journal of allergy, asthma, and immunology, 2016-12, Vol.15 (6), p.498-507
2016
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- Autor(en) / Beteiligte
- Titel
- Metformin Alleviates Lipopolysaccharide-induced Acute Lung Injury through Suppressing Toll-like Receptor 4 Signaling
- Ist Teil von
- Iranian journal of allergy, asthma, and immunology, 2016-12, Vol.15 (6), p.498-507
- Ort / Verlag
- Iran: Tehran University of Medical Sciences
- Erscheinungsjahr
- 2016
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Signaling AMP-activated protein kinase (AMPK), an energy sensing enzyme, has been implicated in controlling inflammation. In this study we investigated whether activation of AMPK by metformin could protect the lung from lipopolysaccharide (LPS)-induced acute injury by inhibitingng TLR4 pathway. Male Wistar rats were randomly divided into 3 groups (n=6): control group received normal saline (0.5 mL), LPS group received LPS (0.5 mg/kg), and metformin-treated group received LPS and metformin (100 mg/kg). Nine hours later nuclear factor-κB (NF-κB), phosphorylated, and non-phosphorylated AMPK using western blot, and the rate of TLR4 mRNA expression using real-time PCR were assessed in the lung tissue. To evaluate neutrophil infiltration, the myeloperoxidase (MPO) activity was measured. The severity of the lung damage was assessed by histological examinations. It was found that the ratio of p-AMPKα to AMPKα was significantly upregulated by 22% (p<0.05) in the lungs obtained from the metformin group. In LPS-treated rats, we observed a high expression of TLR4 in the tissue along with increased levels of MyD88, NF-κB, and TNFα. Metformin considerably reduced all these parameters. Histological examinations revealed that metformin remarkably attenuated congestion and inflammatory cellular infiltration into the alveolar walls and also decreased MPO activity by 37% (p<0.05). We conclude that metformin could protect the lung tissue against LPS-evoked TLR4 activation and the protective effect can be related to the activation of AMPK.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1735-1502eISSN: 1735-5249Titel-ID: cdi_proquest_miscellaneous_1877813082
- Format
- –
- Schlagworte
- Acute Lung Injury - chemically induced, Acute Lung Injury - metabolism, Acute Lung Injury - pathology, Alveoli, AMP-activated protein kinase, AMP-Activated Protein Kinases - drug effects, AMP-Activated Protein Kinases - metabolism, Animals, Antidiabetics, Blotting, Western, Cytokines, Gene expression, Hypoglycemic Agents - pharmacology, Immunoglobulins, Infiltration, Inflammation, Kinases, Laboratory animals, Leukocytes (neutrophilic), Lipopolysaccharides, Lipopolysaccharides - toxicity, Lung - drug effects, Lung - metabolism, Lung - pathology, Lungs, Male, Metformin, Metformin - pharmacology, MyD88 protein, Myeloid Differentiation Factor 88 - drug effects, Myeloid Differentiation Factor 88 - metabolism, Neutrophils, NF-kappa B - drug effects, NF-kappa B - metabolism, NF-κB protein, Peroxidase, Peroxidase - metabolism, Proteins, Rats, Rats, Wistar, Real-Time Polymerase Chain Reaction, Recruitment, RNA, Messenger - drug effects, RNA, Messenger - metabolism, Rodents, Sepsis, Studies, TLR4 protein, Toll-Like Receptor 4 - drug effects, Toll-Like Receptor 4 - genetics, Toll-Like Receptor 4 - metabolism, Toll-like receptors, Transcription factors, Tumor Necrosis Factor-alpha - drug effects, Tumor Necrosis Factor-alpha - metabolism, Tumor necrosis factor-TNF