Journal of applied microbiology, 2002, Vol.92 (1), p.32-40
2002
Details
- Autor(en) / Beteiligte
- Titel
- Aerobic and facultatively anaerobic cellulolytic bacteria from the gut of the termite Zootermopsis angusticollis
- Ist Teil von
- Journal of applied microbiology, 2002, Vol.92 (1), p.32-40
- Ort / Verlag
- Oxford UK: Blackwell Science Ltd
- Erscheinungsjahr
- 2002
- Link zum Volltext
- Quelle
- Wiley Online Library Journals Frontfile Complete
- Beschreibungen/Notizen
- Aims: To demonstrate the occurrence of cellulolytic bacteria in the termite Zootermopsis angusticollis. Methods and Results: Applying aerobic cultivation conditions we isolated 119 cellulolytic strains from the gut of Z. angusticollis, which were assigned to 23 groups of aerobic, facultatively anaerobic or microaerophilic cellulolytic bacteria. 16S rDNA restriction fragment pattern and partial 16S rDNA sequence analysis, as well as numerical taxonomy, were used for the assignment of the isolates. The Gram-positive bacteria of the actinomycetes branch could be assigned to the order Actinomycetales including the genera Cellulomonas/Oerskovia, Microbacterium and Kocuria. The Gram-positive bacteria from the order Bacillales belonged to the genera Bacillus, Brevibacillus and Paenibacillus. Isolates related to the genera Afipia, Agrobacterium/Rhizobium, Brucella/Ochrobactrum, Pseudomonas and Sphingomonas/Zymomonas from the alpha-proteobacteria and Spirosoma-like from the "Flexibacteriaceae" represented the Gram-negative bacteria. Conclusions: A cell titre of up to 10(7) cellulolytic bacteria per ml, determined for some isolates, indicated that they may play a role in cellulose digestion in the termite gut in addition to the cellulolytic flagellates and termite's own cellulases. Significance and Impact of the Study: The impact of bacteria on cellulose degradation in the termite gut has always been a matter of debate. In the present survey we investigated the aerobic and facultatively anaerobic cellulolytic bacteria in the termite gut.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1364-5072eISSN: 1365-2672DOI: 10.1046/j.1365-2672.2002.01502.xTitel-ID: cdi_proquest_miscellaneous_18761999
- Format
- –
- Schlagworte
- Aerobiosis, Afipia, Agrobacterium, Anaerobiosis, Animal, plant and microbial ecology, Animals, Biodegradation, Environmental, Biological and medical sciences, Brevibacillus, Brucella, cellulases, cellulolytic microorganisms, Cellulomonas, cellulose, Cellulose - metabolism, Culture Media, digestion, digestive system, DNA, Ribosomal - analysis, Fundamental and applied biological sciences. Psychology, Gram-negative bacteria, Gram-Negative Bacteria - classification, Gram-Negative Bacteria - genetics, Gram-Negative Bacteria - isolation & purification, Gram-Negative Bacteria - metabolism, Gram-positive bacteria, Gram-Positive Bacteria - classification, Gram-Positive Bacteria - genetics, Gram-Positive Bacteria - isolation & purification, Gram-Positive Bacteria - metabolism, Intestines - microbiology, Isoptera - microbiology, Kocuria, Microbacterium, Microbial ecology, Normal microflora of man and animals. Rumen, nucleotide sequences, numerical taxonomy, Ochrobactrum, Paenibacillus, Pseudomonas, Rhizobium, ribosomal DNA, RNA, Ribosomal, 16S - genetics, sequence analysis, Sequence Analysis, DNA, Sphingomonas, surveys, Zootermopsis angusticollis, Zymomonas