Details

Autor(en) / Beteiligte

• Ye, Xinchun
• Zuo, Dandan
• Yu, Lu
• Zhang, Liang
• Tang, Jiao
• Cui, Chengcheng
• Bao, Lei
• Zan, Kun
• Zhang, Zuohui
• Yang, Xinxin
• Chen, Hao
• Tang, Hai
• Zu, Jie
• Shi, Hongjuan
• Cui, Guiyun

Titel

ROS/TXNIP pathway contributes to thrombin induced NLRP3 inflammasome activation and cell apoptosis in microglia

Ist Teil von

• Biochemical and biophysical research communications, 2017-04, Vol.485 (2), p.499-505

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2017

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• There is no effective therapy for intracerebral hemorrhage (ICH) because of poor understanding of the mechanisms of brain injury after hemorrhage. The NLRP3 inflammasome, as a vital component of innate immune system, which is associated with a wide range of human CNS disorders, including ICH. But its detailed mechanisms in ICH remain mainly unclear. In this study, BV2 cells with thrombin exposure were used to investigate the role of NLRP3 inflammasome in thrombin-induced brain injury. We used western blot to detect NLRP3 inflammasome activation and the expression of thioredoxin binding protein (TXNIP), DCFH-DA to investigate intracellular reactive oxygen species (ROS), flow cytometry to analyze apoptosis. Our results showed that ROS inhibitor N-acetyl-l-cysteine (NAC) suppressed the upregulation of intracellular ROS and TXNIP expression. Furthermore, the cell apoptosis and expression of apoptotic protein were significantly attenuated after treatment of thrombin with NAC or NLRP3 antagonist (MCC950). Thrombin activates ROS/TXNIP/NLRP3 signaling in BV2 cells, which may indicate a mechanism that pro-inflammatory and pro-apoptotic contributes to the development of ICH. •Thrombin induces NLRP3 inflammasome activation in BV2 microglia.•NAC reduced thrombin-induced upregulates ROS and the expression of TXNIP in BV2 microglia.•Inhibition of ROS/TXNIP pathway ameliorates BV2 microglia apoptosis.